Journal: Molecular human reproduction

Article Title: Ectopic endometrial cells express high concentrations of interleukin (IL)-8 in vivo regardless of the menstrual cycle phase and respond to oestradiol by up-regulating IL-1-induced IL-8 expression in vitro.

doi: 10.1093/molehr/7.9.859

Figure Lengend Snippet: Figure 1. Localization of interleukin-8 (IL-8) in endometriotic lesions by immunohistochemistry. Sections were incubated with polyclonal rabbit anti-IL-8 antibody, then with biotin-conjugated anti-rabbit antibody and streptavidin–peroxidase. The immunoreaction was revealed with DAB as chromogen, and haematoxylin which stains the nucleus in blue was used for counterstaining. Note the intense brown positive immunostaining in endometriotic glands and stroma from (A) an ovarian endometriotic lesion, stage IV, proliferative phase, day 4 (patient no. 6, Table I), and (B) the inner lining of ovarian endometrioma, stage IV, secretory phase, day 22 (patient no. 10, Table I). (C and D) control sections incubated with normal rabbit IgG instead of the primary polyclonal rabbit antibody showing no immunostaining. Scale bar 50 µm.

Article Snippet: Immunostaining was performed using a polyclonal rabbit anti-IL-8 antibody [10 μg/ml in PBS containing 1% bovine serum albumin (BSA)] (Biosource International, Camarillo, CA, USA), a biotin-conjugated affinity-purified goat anti-rabbit antibody (H L) (2 μg/ml) (Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA), peroxidase-conjugated streptavidin (1.3 μg/ml) (Jackson ImmunoResearch Laboratories), diaminobenzidine (Sigma Chemical Co, St Louis, MO, USA) as chromogen and haematoxylin for counterstaining.

Techniques: Immunohistochemistry, Incubation, Immunostaining, Control